Landcare and livelihoods: The promotion and adoption of conservation farming systems in the Philippine uplands

Slow adoption of conservation farming systems in the Philippine uplands is a major problem. To address this, the landcare approach, based on community landcare groups, is being piloted in several locations. The results of a study to evaluate the impact of the Landcare Program in Barangay Ned in Southern Mindanao are presented. The sustainable rural livelihoods approach was used as a framework for the evaluation. The study focused on two impacts, the adoption of conservation practices and the formation and development of landcare groups, drawing on four data sources - project reports, key informants, a questionnaire survey, and case studies of landcare groups. The Ned Landcare Program has been associated with rapid adoption of conservation farming practices and the rapid formation of landcare groups linked in an association. The main effect of the Program was to enhance human capital through practical, farmer-led training and extension, enabling farmers to incorporate soil conservation and agroforestry technologies in their farming systems, with desirable outcomes for livelihood security and environmental sustainability. The social capital formed, especially through the landcare association, was crucial to these outcomes. Ongoing partnerships with adequately resourced facilitating agencies were required to maintain the Landcare Program in the long term

Smallholder farmer experience with cassava related pests and diseases and their view on clean/certified planting materials

The recent boom in the global market for cassava has created livelihood opportunities for many smallholders in Southeast Asia. Research over many years by public agencies has generated an abundance of technologies that could enhance the productivity and sustainability of these cassava producers. While national government policies have not prioritised the dissemination of these technologies, we hypothesise that, in particular contexts, private-sector value-chain actors have incentives to invest in the promotion of suitable varieties, fertiliser regimes, pest control methods, and other production practices. In other contexts, however, there is little incentive for private-sector involvement, and support from public-sector or non-government actors will be required. In this paper we briefly analyse the drivers behind the recent boom and provide an overview of smallholder cassava production in the region. We then present a framework to analyse the incentives for private value-chain actors to invest in the promotion of different technologies, taking into account (1) the characteristics of the technology, (2) the nature of the production system, and (3) the features of the value chain, including the ability of value-chain actors to capture the benefits of any investment in technology dissemination. We test this framework through a preliminary analysis of three contrasting case studies: (1) a value chain centred on a large starch factory with a wide catchment area of smallholder suppliers in Dak Lak Province, Vietnam; (2) a cross-border value chain linking smallholders in Kratie Province, Cambodia, to starch factories in Vietnam; and (3) a local value chain for cassava as a food crop in Nusa Tenggara Timur Province, Indonesia

The influence of farmers' mental models on an agroforestry extension program in the Philippines

The influence of farmers' mental models on the success of an agroforestry extension program on Leyte Island in the Philippines was investigated. Knowledge of farmers' mental models and hence the likely acceptance of technology was used to inform the design of a hypothetically expanded program. To gain an insight into the reasons behind differing acceptance of extension assistance, data were collected and analysed from formal interviews, translated conversations and visual observations. The data provided a chain of evidence and triangulation between farmers' stated intentions and their actions. Farmers had little prior knowledge of nursery technology and were highly receptive to extension assistance which enabled them to develop high self-efficacy in seedling production. However, farmers' rejection of silvicultural advice to thin and prune existing plantations was predicated by existing attitudes to forest resource management. Farmers also expressed a strong preference for a low-cost and low-input approach to establishing timber trees. Visual observations of farmers' tree establishment practices indicated the existence of gaps in their knowledge of tree growth processes. This investigation illustrates the need to elicit farmers' mental models as a parallel enquiry to extension activities. If agroforestry extension is to be constructivist and participatory, accommodation of farmers' mental models and modification of program goals may be necessary. Relatively little is known about the reasons for farmers' acceptance or rejection of silviculture in Leyte and these results indicate that further research into the way that farmers' mental models filter and guide acceptance of advice may be worthwhile

C9orf72-ALS human iPSC microglia are pro-inflammatory and toxic to co-cultured motor neurons via MMP9

Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disease characterized by progressive motor neuron loss, with additional pathophysiological involvement of non-neuronal cells such as microglia. The commonest ALS-associated genetic variant is a hexanucleotide repeat expansion (HRE) mutation in C9orf72. Here, we study its consequences for microglial function using human iPSC-derived microglia. By RNA-sequencing, we identify enrichment of pathways associated with immune cell activation and cyto-/chemokines in C9orf72 HRE mutant microglia versus healthy controls, most prominently after LPS priming. Specifically, LPS-primed C9orf72 HRE mutant microglia show consistently increased expression and release of matrix metalloproteinase-9 (MMP9). LPS-primed C9orf72 HRE mutant microglia are toxic to co-cultured healthy motor neurons, which is ameliorated by concomitant application of an MMP9 inhibitor. Finally, we identify release of dipeptidyl peptidase-4 (DPP4) as a marker for MMP9-dependent microglial dysregulation in co-culture. These results demonstrate cellular dysfunction of C9orf72 HRE mutant microglia, and a non-cell-autonomous role in driving C9orf72-ALS pathophysiology in motor neurons through MMP9 signaling

Policies, Political-Economy, and Swidden in Southeast Asia

For centuries swidden was an important farming practice found across the girth of Southeast Asia. Today, however, these systems are changing and sometimes disappearing at a pace never before experienced. In order to explain the demise or transitioning of swidden we need to understand the rapid and massive changes that have and are occurring in the political and economic environment in which these farmers operate. Swidden farming has always been characterized by change, but since the onset of modern independent nation states, governments and markets in Southeast Asia have transformed the terms of swiddeners’ everyday lives to a degree that is significantly different from that ever experienced before. In this paper we identified six factors that have contributed to the demise or transformation of swidden systems, and support these arguments with examples from China (Xishuangbanna), Laos, Thailand, Malaysia, and Indonesia. These trends include classifying swiddeners as ethnic minorities within nation-states, dividing the landscape into forest and permanent agriculture, expansion of forest departments and the rise of conservation, resettlement, privatization and commoditization of land and land-based production, and expansion of market infrastructure and the promotion of industrial agriculture. In addition we note a growing trend toward a transition from rural to urban livelihoods and expanding urban-labor markets

Simulation-based cheminformatic analysis of organelle-targeted molecules: lysosomotropic monobasic amines

Cell-based molecular transport simulations are being developed to facilitate exploratory cheminformatic analysis of virtual libraries of small drug-like molecules. For this purpose, mathematical models of single cells are built from equations capturing the transport of small molecules across membranes. In turn, physicochemical properties of small molecules can be used as input to simulate intracellular drug distribution, through time. Here, with mathematical equations and biological parameters adjusted so as to mimic a leukocyte in the blood, simulations were performed to analyze steady state, relative accumulation of small molecules in lysosomes, mitochondria, and cytosol of this target cell, in the presence of a homogenous extracellular drug concentration. Similarly, with equations and parameters set to mimic an intestinal epithelial cell, simulations were also performed to analyze steady state, relative distribution and transcellular permeability in this non-target cell, in the presence of an apical-to-basolateral concentration gradient. With a test set of ninety-nine monobasic amines gathered from the scientific literature, simulation results helped analyze relationships between the chemical diversity of these molecules and their intracellular distributions